Kobari M, Matsuno S, Sato T, Kan M, Tachibana T
Tohoku J Exp Med. 1984 May;143(1):33-46. doi: 10.1620/tjem.143.33.
We succeeded in an establishment of a human pancreatic cancer cell line (PK-1) from liver metastasis of pancreatic cancer. Primary pancreatic cancer cells grew as islands surrounded by fibroblastic cells. However, these fibroblastic cells were gradually omitted by the polygonal shaped cancer cells. This cell line contained neither zymogen granules nor trypsin indicating that this pancreatic cancer originated from pancreatic duct cells. Modal chromosome numbers of this cell line were 42 and 72 and the doubling time was 48 hr. This cell line was transplantable in athymic nude mice to form progressive tumors which had histology similar to that of the original cancer (papillotubular adenocarcinoma). Neither AFP nor ferritin but CEA was detected on the surface and in the cytoplasm of this cell line in indirect immunofluorescence. Rabbit antiserum against this pancreatic cancer cell line detected pancreatic cancer associated antigen besides CEA in the culture supernatant. This antiserum reacted with sera from patients with pancreatic cancer to form a distinct precipitin line in agarose gel which fused with the precipitin line formed between the culture supernatant of this cell line and the antiserum.
我们成功地从胰腺癌肝转移灶建立了一株人胰腺癌细胞系（PK - 1）。原发性胰腺癌细胞呈岛屿状生长，周围被成纤维细胞包围。然而，这些成纤维细胞逐渐被多边形癌细胞取代。该细胞系既不含酶原颗粒也不含胰蛋白酶，表明这种胰腺癌起源于胰腺导管细胞。该细胞系的众数染色体数为42和72，倍增时间为48小时。该细胞系可移植到无胸腺裸鼠体内形成进行性肿瘤，其组织学与原发癌（乳头管状腺癌）相似。在间接免疫荧光中，该细胞系的表面和细胞质中未检测到甲胎蛋白和铁蛋白，但检测到癌胚抗原。兔抗该胰腺癌细胞系血清在培养上清液中除癌胚抗原外还检测到胰腺癌相关抗原。该抗血清与胰腺癌患者的血清反应，在琼脂糖凝胶中形成一条明显的沉淀线，该沉淀线与该细胞系培养上清液和抗血清之间形成的沉淀线融合。
