Kobari M, Hisano H, Matsuno S, Sato T, Kan M, Tachibana T
Tohoku J Exp Med. 1986 Nov;150(3):231-48. doi: 10.1620/tjem.150.231.
Six human pancreatic cancer cell lines PK-1, -8, -9, -12, -14 and -16, were established. They originated from either primary pancreatic cancer biopsy or liver metastasis biopsy, or xenografts of these biopsy specimens in athymic nude mice. The primary tumors were all well differentiated adenocarcinomas of pancreatic duct origin. The six established PK cell lines were all CEA positive and had tumorigenicity in athymic nude mice. Morphology of the xenografted tumors was closely similar to that of the original tumor. PK cells grew slowly with the doubling time of 41.3 to 82 hr and showed aneuploid chromosome pattern. High levels of glucose-6-phosphate dehydrogenase (G6PDH) and lactic dehydrogenase (LDH) were found in each cell extract. Trypsin was not detected in cell extracts except PK-8 and PK-9. In chemosensitivity test, all of PK cell lines were sensitive to aclacinomycin A (ACM), and PK-1 and PK-8 were sensitive to 5-Fluorouracil (5-Fu) at concentrations of 0.02 microgram/ml, ACM and 1 microgram/ml, 5-Fu, when the drugs were used for over 48 hr. At higher concentrations, they showed time independent sensitivity to mitomycin C (MMC). PK-9 was resistant to 5-Fu and MMC.
建立了六种人胰腺癌细胞系PK - 1、- 8、- 9、- 12、- 14和- 16。它们来源于原发性胰腺癌活检组织、肝转移活检组织，或这些活检标本在无胸腺裸鼠体内的异种移植瘤。原发性肿瘤均为起源于胰腺导管的高分化腺癌。所建立的六种PK细胞系均CEA阳性，且在无胸腺裸鼠体内具有致瘤性。异种移植瘤的形态与原发肿瘤极为相似。PK细胞生长缓慢，倍增时间为41.3至82小时，呈现非整倍体染色体模式。在每个细胞提取物中均发现高水平的葡萄糖-6-磷酸脱氢酶（G6PDH）和乳酸脱氢酶（LDH）。除PK - 8和PK - 9外，在细胞提取物中未检测到胰蛋白酶。在化学敏感性试验中，所有PK细胞系对阿克拉霉素A（ACM）敏感，当药物作用超过48小时时，PK - 1和PK - 8对浓度为0.02微克/毫升的ACM和1微克/毫升的5-氟尿嘧啶（5-Fu）敏感。在较高浓度下，它们对丝裂霉素C（MMC）表现出与时间无关的敏感性。PK - 9对5-Fu和MMC耐药。
