α-1-抗胰蛋白酶苏木精和伊红荧光法：一种快速、实用的筛查技术。

Alpha-1-antitrypsin hematoxylin and eosin fluorescence: a rapid, useful screening technic.

作者信息

Dervan P A

出版信息

Am J Clin Pathol. 1984 Aug;82(2):188-90. doi: 10.1093/ajcp/82.2.188.

DOI:10.1093/ajcp/82.2.188

PMID:6205582

Abstract

Sections stained with hematoxylin and eosin from seven liver biopsies and two ovarian mixed mesodermal tumors containing alpha-1-antitrypsin globules were examined by fluorescent microscopy. Al-AT globules readily were seen in all biopsies and fluoresced brightly. Megamitochondria and acidophil bodies in liver biopsies also fluoresced but were distinguished easily by their morphology and topographic location. Normal liver biopsies contained no fluorescent bodies. It is concluded that examination of routine hematoxylin and eosin stained sections by fluorescent microscopy is a rapid convenient screening method for the detection of al-AT globules. Acidophil bodies and megamitochondria also are seen by this method.

摘要

对七份肝活检组织以及两份含有α-1-抗胰蛋白酶小球的卵巢混合性中胚层肿瘤进行苏木精和伊红染色切片，通过荧光显微镜检查。在所有活检组织中均能轻易看到α-1-抗胰蛋白酶小球，且发出明亮荧光。肝活检组织中的巨型线粒体和嗜酸性小体也能发出荧光，但可通过其形态和位置轻松区分。正常肝活检组织中不含荧光体。结论是，通过荧光显微镜检查常规苏木精和伊红染色切片是检测α-1-抗胰蛋白酶小球的一种快速便捷的筛查方法。通过这种方法也能看到嗜酸性小体和巨型线粒体。