Neumann P E, Horoupian D S, Goldman J E, Hess M A
Am J Pathol. 1984 Aug;116(2):214-22.
Crooke's hyaline change was studied by immunocytochemistry using an anti-adrenocorticotropic hormone (ACTH) antiserum and five different antisera against cytokeratins. Crooke's hyaline appears in basophil cells of the adenohypophysis in patients with hypercortisolism, presumably as a part of the negative feedback on corticotropin secretion. Previous studies have identified the hyaline material as a simple protein, apparently unrelated to ACTH, and electron microscopy has revealed a loss of secretory granules and an accumulation of 6-9-nm filaments in the cytoplasm of affected cells. In this study, the secretory granules in adenohypophysial cells exhibiting Crooke's hyaline change were labeled by anti-ACTH antibodies, while the hyaline material was positive for cytokeratin with each of the five antisera used. The results suggest that high levels of glucocorticoids may stimulate elaboration of cytokeratins in basophils while they suppress the production and release of ACTH.
运用抗促肾上腺皮质激素（ACTH）抗血清和五种不同的抗细胞角蛋白抗血清，通过免疫细胞化学方法对克罗克透明变性进行了研究。克罗克透明变性出现在皮质醇增多症患者腺垂体的嗜碱性细胞中，推测这是对促肾上腺皮质激素分泌的负反馈的一部分。先前的研究已将这种透明物质鉴定为一种简单蛋白质，显然与促肾上腺皮质激素无关，并且电子显微镜检查显示受影响细胞的细胞质中分泌颗粒减少，出现6 - 9纳米细丝的积聚。在本研究中，呈现克罗克透明变性的腺垂体细胞中的分泌颗粒被抗促肾上腺皮质激素抗体标记，而透明物质在用的五种抗血清中对细胞角蛋白均呈阳性。结果表明，高水平的糖皮质激素可能刺激嗜碱性细胞中细胞角蛋白的合成，同时抑制促肾上腺皮质激素的产生和释放。
