Meyer E, Caron F, Guiard B
Biochimie. 1984 May;66(5):403-12. doi: 10.1016/0300-9084(84)90024-5.
Paramecium primaurelia mRNAs were translated in vitro in rabbit reticulocyte lysate and the products of translation were analyzed by their size. We show that the large majority of these products are of short but discrete sizes irrespective of the length of the mRNA which directs their synthesis. An illustrative example is given by the translation of mRNA of G surface antigen which directs the synthesis of a 50 kD polypeptide instead of the complete 250 kD protein. Control experiments suggest that the blocking is due to mRNA primary structure.
将嗜热四膜虫的mRNA在兔网织红细胞裂解物中进行体外翻译，并根据翻译产物的大小对其进行分析。我们发现，这些产物中的绝大多数大小较短但离散，与指导其合成的mRNA长度无关。一个说明性的例子是G表面抗原mRNA的翻译，它指导合成一种50 kD的多肽，而不是完整的250 kD蛋白质。对照实验表明，这种阻断是由于mRNA的一级结构所致。
