Charnay P, Treisman R, Mellon P, Chao M, Axel R, Maniatis T
Cell. 1984 Aug;38(1):251-63. doi: 10.1016/0092-8674(84)90547-6.
Human beta-globin genes introduced into mouse erythroleukemia (MEL) cells by DNA cotransformation are correctly regulated when erythroid cell differentiation is induced by dimethylsulfoxide (DMSO). In contrast, cloned human alpha-globin genes are efficiently transcribed in MEL cells prior to induction, and no increase in the level of alpha-globin mRNA is observed when the cells differentiate. These observations suggest that the mechanisms by which alpha- and beta-globin genes are activated during erythroid cell differentiation are fundamentally different. Analysis of the transcription of hybrid human alpha/beta-globin genes in MEL cells revealed that the sequences responsible for differences in transcription of the intact alpha- and beta-globin genes are located on the 3' side of the mRNA capping site of the two genes, suggesting that cis-acting regulatory sequences are located within the structural genes.
通过DNA共转化导入小鼠红白血病（MEL）细胞的人β-珠蛋白基因，在由二甲基亚砜（DMSO）诱导红系细胞分化时受到正确调控。相比之下，克隆的人α-珠蛋白基因在诱导之前就在MEL细胞中高效转录，并且当细胞分化时未观察到α-珠蛋白mRNA水平的增加。这些观察结果表明，α-和β-珠蛋白基因在红系细胞分化过程中被激活的机制根本不同。对MEL细胞中杂交人α/β-珠蛋白基因转录的分析表明，负责完整α-和β-珠蛋白基因转录差异的序列位于这两个基因mRNA帽位点的3'侧，这表明顺式作用调控序列位于结构基因内。
