Pestana de Castro A F, Perreau P, Rodrigues A C, Simoes M
Ann Microbiol (Paris). 1980 May-Jun;131(3):255-63.
Thirty-one type A strains of Pasteurella multocida isolated from rabbits and poultry were able to agglutinate red blood cells (RBC) from human group O donors. Except for human RBC from group B which were also agglutinated by 3 strains, neither group A nor RBC from sheep and rabbits were agglutinated. Haemagglutination was mannose-resistant, and the two techniques used detected unrelated activities. One as tested by a slide haemagglutination (MRSH) test was common to most (31 out of 34 strains) capsulated strains, though non-mucoid ones obtained by growth on hyaluronidase-containing medium showed also this property. This haemagglutination activity was destroyed at 100 degrees C for 15 min but not at 56 degrees C for 30 min, was dependent upon an optimal pH range (7.4-7.5) and did not seem to be influenced by different enriched media. The other activity was assayed by the microhaemagglutination (MRMH) test. Only part (19 out of 34 strains) of the strains presented this activity, which was also destroyed by boiling for 15 min and was dependent on the addition of 0.5 of polyvinilpirrolidone to phosphate buffered saline, used to suspend RBC. A correlation between haemagglutination activities of P. multocida type A and the possibility of this microorganism harbouring fimbriate organelles is discussed.
从兔和家禽中分离出的31株A型多杀性巴氏杆菌能够凝集来自O型血供者的人红细胞（RBC）。除了3株菌株也能凝集B型人红细胞外，A型人红细胞以及绵羊和兔的红细胞均未被凝集。血凝反应对甘露糖具有抗性，所使用的两种技术检测到不相关的活性。一种通过玻片血凝试验（MRSH）检测，大多数（34株中的31株）荚膜菌株都有这种活性，不过在含透明质酸酶的培养基上生长获得的非黏液型菌株也具有这种特性。这种血凝活性在100℃下15分钟被破坏，但在56℃下30分钟未被破坏，依赖于最佳pH范围（7.4 - 7.5），似乎不受不同富集培养基的影响。另一种活性通过微量血凝试验（MRMH）检测。只有部分菌株（34株中的19株）呈现这种活性，该活性也在煮沸15分钟后被破坏，并且依赖于向用于悬浮红细胞的磷酸盐缓冲盐水中添加0.5的聚乙烯吡咯烷酮。讨论了A型多杀性巴氏杆菌的血凝活性与该微生物携带菌毛细胞器可能性之间的相关性。
