Frederiks W M, Fronik G M, Hesseling J M
Exp Mol Pathol. 1984 Aug;41(1):119-25. doi: 10.1016/0014-4800(84)90012-1.
A method has been developed for quantitative analysis of necrotic regions in lobes of the rat liver 24 hr after temporary ischemia. Essentially the method consists of cutting the liver lobes into 2-mm-thick slices by means of a specially developed cutting apparatus. The serial slices are incubated in a special holder with tetranitro BT, which enables a clear discrimination between necrotic (unstained) and undamaged (stained) tissue. All slices from the ischemic lobes are photographed in the holder and submitted to morphometric analysis on enlarged prints. This method to obtain an estimate of the volume density of necrotic regions is rapid, simple, and reliable and compares favorably with other methods used in the literature. The extent of necrosis after 15-min clamping of the afferent vessels is virtually nil; it rises with longer periods of necrosis to reach a value of +/- 80% after 120 min.
已开发出一种方法，用于对大鼠肝脏叶在短暂缺血24小时后的坏死区域进行定量分析。该方法主要包括通过专门开发的切割装置将肝叶切成2毫米厚的切片。将连续切片置于含有四硝基BT的特殊支架中孵育，这使得能够清晰区分坏死（未染色）组织和未受损（染色）组织。对缺血肝叶的所有切片在支架中进行拍照，并在放大的照片上进行形态计量分析。这种获得坏死区域体积密度估计值的方法快速、简单且可靠，与文献中使用的其他方法相比具有优势。传入血管夹闭15分钟后的坏死程度几乎为零；随着坏死时间延长，坏死程度上升，在120分钟后达到约±80%的值。
