Mattes M J, Cairncross J G, Old L J, Lloyd K O
Hybridoma. 1983;2(3):253-64. doi: 10.1089/hyb.1983.2.253.
Four mouse monoclonal antibodies to widely distributed human cell surface antigens are described. The AJ2 antigen is one of the immunodominant antigens in mice immunized with human cells, as demonstrated by testing conventional mouse antisera. This antigen has four subunits having molecular weights (MW) of 170 kD, 140 kD, 140 kD, and 28 kD when immunoprecipitated from the immunizing cell line, an astrocytoma. With other cell lines the molecular weights and subunit composition varies, but the major component is always of MW 125-140 kD. The antigen was detected on all human tissue culture cells and on sections of all normal human tissues tested. Antibody MA99 detects the same antigenic complex but shows a slightly different pattern of reactivity with cell lines and tissues. The MA103 antigen has a similar ubiquitous distribution. It is a glycoprotein of MW 50-55 kD and the determinant detected is not denatured by treatment at 100 degrees C. MH99, the third common antigen detected, has two glycosylated subunits with MW of 29 kD and 38 kD. It is present on a subpopulation of tumor cell lines of most histologic types except that it is absent or weakly expressed on melanomas and astrocytomas. MH99 is present on some T lymphomas and leukemias and myeloid leukemias but was not detected on B lymphomas, null cell lymphomas and myelomas. It was detected preferentially on epithelial cells in tissue sections.
本文描述了四种针对广泛分布的人类细胞表面抗原的小鼠单克隆抗体。AJ2抗原是用人细胞免疫的小鼠中的主要免疫显性抗原之一，这已通过检测传统小鼠抗血清得到证实。从免疫细胞系（一种星形细胞瘤）免疫沉淀时，该抗原具有四个亚基，分子量（MW）分别为170 kD、140 kD、140 kD和28 kD。对于其他细胞系，分子量和亚基组成会有所不同，但主要成分的分子量始终在125 - 140 kD之间。在所有人类组织培养细胞以及所有测试的正常人类组织切片上均检测到了该抗原。抗体MA99检测到相同的抗原复合物，但与细胞系和组织的反应模式略有不同。MA103抗原具有类似的广泛分布。它是一种分子量为50 - 55 kD的糖蛋白，所检测到的决定簇在100℃处理时不会变性。检测到的第三种常见抗原MH99有两个糖基化亚基，分子量分别为29 kD和38 kD。它存在于大多数组织学类型的肿瘤细胞系亚群中，但在黑色素瘤和星形细胞瘤中不存在或弱表达。MH99存在于一些T淋巴瘤、白血病和髓系白血病中，但在B淋巴瘤、裸细胞淋巴瘤和骨髓瘤中未检测到。在组织切片中，它优先在上皮细胞上被检测到。
