McGuire T C, Palmer G H, Goff W L, Johnson M I, Davis W C
Infect Immun. 1984 Sep;45(3):697-700. doi: 10.1128/iai.45.3.697-700.1984.
Anaplasma marginale-infected erythrocytes were examined for the presence of maturation, isolate-restricted, and isolate-common antigens by indirect immunofluorescence with monoclonal antibodies. A panel of 18 monoclonal antibodies was used; none of the antibodies reacted with Anaplasma ovis, Babesia bigemina, babesia bovis, Trypanosoma brucei, Trypanosoma congolense, or uninfected bovine erythrocytes. Antigens common to all six A. marginale isolates were detected by nine antibodies. Single isolates from Florida, Southern Idaho, Northern Texas, and Virginia and two isolates from Washington state had four patterns of reactivity with a second panel of nine antibodies. Antigenically distinct stages were not detected, as sequential smears taken daily during acute infection had the same pattern of reactivity. The results demonstrate antigenic heterogeneity among isolates of A. marginale and the presence of common antigens. This information allows grouping of different isolates and, more importantly, provides a method for the identification and isolation of common antigens for diagnostic tests.
利用单克隆抗体通过间接免疫荧光法检测边缘无形体感染的红细胞中成熟抗原、分离株特异性抗原和分离株共同抗原的存在情况。使用了一组18种单克隆抗体；没有一种抗体与绵羊无形体、双芽巴贝斯虫、牛巴贝斯虫、布氏锥虫、刚果锥虫或未感染的牛红细胞发生反应。9种抗体检测到了所有6种边缘无形体分离株共有的抗原。来自佛罗里达州、爱达荷州南部、得克萨斯州北部和弗吉尼亚州的单个分离株以及来自华盛顿州的两个分离株与第二组9种抗体有四种反应模式。未检测到抗原性不同的阶段，因为在急性感染期间每天采集的连续涂片具有相同的反应模式。结果表明边缘无形体分离株之间存在抗原异质性以及共同抗原的存在。这些信息有助于对不同分离株进行分组，更重要的是，提供了一种鉴定和分离用于诊断测试的共同抗原的方法。
