Nakatani H, Tanaka A, Hiromi K
J Appl Biochem. 1983 Dec;5(6):371-4.
Using an ultrafiltration cell, the continuous production of maltose and a mixture of other oligosaccharides was achieved by the combined action of alpha- or beta-amylase and phosphorylase. For the beta-amylase-phosphorylase system, a steady production of maltose was obtained until the beta-amylase was inactivated. For the alpha-amylase-phosphorylase system, it was difficult to maintain a stable production of oligosaccharides, probably due to the destruction of substrate glycogen by random cleavage by alpha-amylase.
使用超滤池,通过α-淀粉酶或β-淀粉酶与磷酸化酶的联合作用实现了麦芽糖和其他寡糖混合物的连续生产。对于β-淀粉酶-磷酸化酶系统,在β-淀粉酶失活之前可获得稳定的麦芽糖产量。对于α-淀粉酶-磷酸化酶系统,难以维持寡糖的稳定生产,这可能是由于α-淀粉酶随机切割导致底物糖原被破坏。