Vethamany-Globus S, Globus M, Darch A, Milton G, Tomlinson B L
J Exp Zool. 1984 Jul;231(1):63-74. doi: 10.1002/jez.1402310109.
This work provides data demonstrating a stimulatory effect of insulin on macromolecular events occurring in cultured regeneration blastemata and demonstrates a synergistic interdependence between nerves and insulin in newt limb regeneration. The current experiments provide evidence for the following: (1) Insulin is paramount for expression of the mitogenic effect of nerves on cultures blastemata. (2) Insulin stimulates the incorporation of (3H)uridine into the acid-insoluble fraction of blastemal homogenates, but it does not alter the turnover rate of incorporated labeled uridine. (3) Insulin also stimulates the incorporation of 35SO4 and (3H)leucine into both chondroitinase-sensitive and chondroitinase-resistant blastemal proteoglycans. (4) Insulin increases the uptake of radiolabeled precursors by the blastemata, namely, (3H)leucine, (3H)uridine, 35SO4, (3H)alpha-aminoisobutyrate, and (3H)2-deoxy-D-glucose. The importance of insulin in the regulation of newt limb regeneration is discussed.
这项工作提供的数据表明胰岛素对培养的再生芽基中发生的大分子事件具有刺激作用，并证明了蝾螈肢体再生中神经与胰岛素之间存在协同相互依存关系。当前的实验为以下内容提供了证据：（1）胰岛素对于神经对培养芽基的促有丝分裂作用的表达至关重要。（2）胰岛素刺激（3H）尿苷掺入芽基匀浆的酸不溶部分，但它不会改变掺入的标记尿苷的周转率。（3）胰岛素还刺激35SO4和（3H）亮氨酸掺入对软骨素酶敏感和对软骨素酶不敏感的芽基蛋白聚糖中。（4）胰岛素增加芽基对放射性标记前体的摄取，即（3H）亮氨酸、（3H）尿苷、35SO4、（3H）α-氨基异丁酸和（3H）2-脱氧-D-葡萄糖。文中讨论了胰岛素在蝾螈肢体再生调节中的重要性。
