Bao C Y, Singer M, Ilan J
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7971-4. doi: 10.1073/pnas.83.20.7971.
Analysis of the effects of forelimb amputation and denervation on [35S]methionine incorporation into the protein of newt brachial plexus nerve ganglia showed that amputation increases the level of protein synthesis about 4-fold as compared with that of control (nonamputated) animals. Denervation without amputation nearly doubles the level of protein synthesis as compared with controls. Analysis of labeled protein by means of two-dimensional gel electrophoresis and radioautogram revealed incorporation patterns that are similar to controls; this observation suggests that amputation affects nerve cells quantitatively rather than qualitatively, in influencing the production of putative neurotrophic agents. A group of basic proteins ranging in Mr from 15,000 to 31,000 are prominently expressed in these radioautograms from experimental ganglia and may be important in promoting forelimb regeneration in the newt.
对蝾螈臂丛神经节蛋白质中[35S]甲硫氨酸掺入情况进行的前肢截肢和去神经支配效应分析表明，与对照（未截肢）动物相比，截肢使蛋白质合成水平增加了约4倍。未截肢的去神经支配使蛋白质合成水平比对照增加了近一倍。通过二维凝胶电泳和放射自显影对标记蛋白质进行分析，结果显示其掺入模式与对照相似；这一观察结果表明，截肢在影响假定神经营养因子产生方面，对神经细胞的影响是定量的而非定性的。一组分子量在15,000至31,000之间的碱性蛋白质在实验神经节的这些放射自显影片中显著表达，可能对促进蝾螈前肢再生很重要。
