Jacobs D M
Rev Infect Dis. 1984 Jul-Aug;6(4):501-5. doi: 10.1093/clinids/6.4.501.
A sensitive hapten-sandwich immunofluorescence technique was used to examine binding of lipopolysaccharide (LPS) at the single-cell level. The structural features governing such binding to murine lymphocytes were investigated by evaluating LPS binding in the presence of a variety of charged molecules and after different target-cell treatments. Polymyxin B, the positively charged proteins egg-white lysozyme and protamine chloride, and the polyanion dextran sulfate inhibited LPS binding to murine lymphocytes. Pretreatment with the proteolytic enzyme pronase and the cross-linking agent paraformaldehyde abolished the capacity of lymphocytes to bind LPS. Inhibition by polymyxin B was less effective when added 30 min after initiation of incubation of LPS with cells at 0 C or when added at the initiation of incubation at 37 C. These results suggest that the interaction between LPS and lymphocytes is a two-stage process, the first of which is dependent on a positively charged membrane protein. The second stage is postulated to be an irreversible hydrophobic interaction between LPS and membrane lipids.
采用一种灵敏的半抗原夹心免疫荧光技术在单细胞水平检测脂多糖（LPS）的结合情况。通过评估在多种带电分子存在时以及不同靶细胞处理后的LPS结合情况，研究了决定这种结合至小鼠淋巴细胞的结构特征。多粘菌素B、带正电荷的蛋白质蛋清溶菌酶和氯化鱼精蛋白以及多阴离子硫酸葡聚糖均抑制LPS与小鼠淋巴细胞的结合。用蛋白水解酶链霉蛋白酶和交联剂多聚甲醛预处理可消除淋巴细胞结合LPS的能力。当在0℃将LPS与细胞孵育开始30分钟后添加多粘菌素B时，或在37℃孵育开始时添加时，其抑制效果较差。这些结果表明，LPS与淋巴细胞之间的相互作用是一个两阶段过程，第一阶段依赖于带正电荷的膜蛋白。第二阶段推测为LPS与膜脂之间不可逆的疏水相互作用。
