Liang R T, Shi Y X
Sci Sin B. 1984 Mar;27(3):257-64.
The lactose binding agglutination factor from rabbit skeletal muscle is isolated through the use of lactose and urea. The factor is purified with DEAE cellulose and Sepharose 4B chromatography. The molecular weight of the factor is determined with Sephadex G-75 chromatography and found to be 28,000 Daltons. The subunit's molecular weight is determined by SDS gel electrophoresis, and found to be 14,000 Daltons. It was found that this binding factor can agglutinate trypsin-treated rabbit erythrocytes. Moreover, this agglutination is inhibited by EDTA and lactose.
通过使用乳糖和尿素从兔骨骼肌中分离出乳糖结合凝集因子。该因子用DEAE纤维素和琼脂糖4B色谱法进行纯化。用葡聚糖G-75色谱法测定该因子的分子量，发现为28,000道尔顿。通过SDS凝胶电泳测定亚基的分子量，发现为14,000道尔顿。发现这种结合因子可以凝集经胰蛋白酶处理的兔红细胞。此外，这种凝集受到EDTA和乳糖的抑制。
