Sjöberg P O, Lindahl M, Porath J, Wadström T
Institute of Biochemistry, Uppsala University, Sweden.
Biochem J. 1988 Oct 1;255(1):105-11. doi: 10.1042/bj2550105.
CS2 fimbriae of enterotoxigenic Escherichia coli were purified and characterized. The surface haemagglutinins (fimbriae) were detached by sonication from a strain producing only the CS2 fimbriae. Isolation was carried out by gel filtration on a Sepharose 4B column. After depolymerization, the fimbriae subunits were purified on a Sephacryl S-300 column in 8.0 M-guanidinium chloride. From 1 litre of medium, 4-6 mg of purified fimbriae was obtained. We found that CS2 fimbriae were completely dissociated by saturated guanidinium chloride into subunits with a molecular mass of 16.5 kDa. CS2 fimbriae was sialic acid-specific, since sialic acids were the most potent inhibitors, and neuraminidase treatment of erythrocytes abolished haemagglutination. Both fimbriae and fimbrial subunits were found to bind to bovine erythrocytes. The binding of subunits to erythrocytes could be inhibited with low concentrations of sialyl-lactose.
对产肠毒素大肠杆菌的CS2菌毛进行了纯化和特性鉴定。通过超声处理从仅产生CS2菌毛的菌株中分离出表面血凝素（菌毛）。通过在琼脂糖4B柱上进行凝胶过滤进行分离。解聚后，菌毛亚基在含8.0M氯化胍的Sephacryl S - 300柱上纯化。从1升培养基中获得了4 - 6毫克纯化菌毛。我们发现CS2菌毛被饱和氯化胍完全解离成分子量为16.5 kDa的亚基。CS2菌毛具有唾液酸特异性，因为唾液酸是最有效的抑制剂，用神经氨酸酶处理红细胞可消除血凝作用。发现菌毛和菌毛亚基均能与牛红细胞结合。低浓度的唾液酸乳糖可抑制亚基与红细胞的结合。
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