Wise K S, Watson R K
Yale J Biol Med. 1983 Sep-Dec;56(5-6):623-9.
A library has been constructed of approximately 50 monoclonal antibodies that recognize antigens of Mycoplasma hyorhinis. Characteristics of six antibodies are discussed. Each reacts with a discrete determinant borne on a protein-containing molecule of distinct molecular size. Three of these respective antigens are expressed at the surface of mycoplasmas colonizing lymphoblastoid cells in culture. Of these three surface antigens, two are selectively expressed on strain GDL but not on strain BTS-7 of M. hyorhinis, thus defining strain-restricted immunological specificities within these species. One monoclonal antibody of the IgM class (mu, kappa) mediated marked complement-dependent growth inhibition of M. hyorhinis in broth culture. These monoclonal reagents should facilitate analysis of mycoplasma surface architecture, and the molecular interactions of these organisms with the host cell surface.
已构建了一个由约50种识别猪鼻支原体抗原的单克隆抗体组成的文库。讨论了六种抗体的特性。每种抗体都与不同分子大小的含蛋白质分子上携带的离散决定簇发生反应。其中三种各自的抗原在培养中定殖于淋巴母细胞样细胞的支原体表面表达。在这三种表面抗原中，有两种在猪鼻支原体GDL株上选择性表达，而在BTS - 7株上不表达，从而在这些物种中定义了菌株限制性免疫特异性。一种IgM类（μ，κ）单克隆抗体在肉汤培养中介导了对猪鼻支原体的显著补体依赖性生长抑制。这些单克隆试剂应有助于分析支原体表面结构以及这些生物体与宿主细胞表面的分子相互作用。
