Ellisor S S, O'Day T, Reid M E, Arndt L, Sugasawara E
Am J Clin Pathol. 1984 Oct;82(4):439-41. doi: 10.1093/ajcp/82.4.439.
Kell null red blood cell samples can be prepared using 2-aminoethylisothiouronium bromide (AET). This article describes a modification whereby only three drops of a 5% red blood cell suspension may be AET treated. This procedure has been used routinely in the authors' laboratory for more than a year. One patient's serum contained anti-Kpb plus anti-C and anti-D. Tests with panel cells pretreated with AET made it possible to identify underlying Rh antibodies without using a panel of genetic Kpb negative red blood cells. Of 24 red blood cell eluates from patient sample with warm autoantibodies, one had specificity within the Kell blood group system. This small volume AET-treatment method is a quick screen for the differentiation of a Kell-related specificity from a non-Kell specificity of both warm autoantibodies and alloantibodies to high-incidence antigens.
凯尔血型系统零型红细胞样本可使用2-氨基乙基异硫脲溴化物（AET）制备。本文描述了一种改进方法，即仅用三滴5%红细胞悬液进行AET处理。该程序已在作者实验室常规使用一年多。一名患者的血清中含有抗-Kpb以及抗-C和抗-D。用AET预处理的谱细胞进行检测，使得无需使用一组基因Kpb阴性红细胞就能鉴定出潜在的Rh抗体。在24份来自伴有温自身抗体的患者样本的红细胞洗脱液中，有一份在凯尔血型系统内具有特异性。这种小体积AET处理方法是一种快速筛查方法，用于区分温自身抗体和针对高频率抗原的同种抗体中与凯尔相关的特异性和非凯尔特异性。
