Winkler M M, Beattie K M, Cisco S L, Sigmund K E, Johnson C L, Rabin B I, Marsh W L
American Red Cross Blood Services, Southeastern Michigan Region, Detroit.
Transfusion. 1989 Sep;29(7):642-5. doi: 10.1046/j.1537-2995.1989.29789369686.x.
This report describes a healthy blood donor whose red cells have weakened expression of Kell blood group antigens. Kell antigen activity could not be detected by flow cytometric analysis and was demonstrable only by sensitive serologic techniques. As with normal-strength Kell antigens, reactivity could be abolished by treatment with 2-aminoethylisothiouronium bromide (AET). The donor's red cells have Kx antigen activity. Other commonly tested blood group antigens (MNSs, Rh, P1, Lewis, Duffy, and Kidd systems) appear normal. Clinical and serologic examination showed that this case is different from previously described examples of modified Kell expression. The propositus's phenotype has remained unchanged for 19 months, which suggests that it is not a transient condition. However, family studies provide no evidence that it is inherited. A 93-kD protein, which reacted weakly by Western blot with rabbit antibody to Kell protein, was isolated from the propositus's red cells by immunoprecipitation. This finding was not reproduced in subsequent studies, which suggests that the quantity of Kell protein recovered was at the threshold level detectable by the technique used. The red cell phenotype is categorized as Kmod, of which this is the first example reported in a healthy individual.
本报告描述了一名健康的献血者，其红细胞上凯尔血型抗原的表达减弱。通过流式细胞术分析无法检测到凯尔抗原活性，只有采用灵敏的血清学技术才能证实其存在。与正常强度的凯尔抗原一样，用2-氨基乙基异硫脲溴化物（AET）处理可消除其反应性。该献血者的红细胞具有Kx抗原活性。其他常见检测的血型抗原（MNSs、Rh、P1、Lewis、达菲和基德系统）看起来正常。临床和血清学检查表明，该病例与先前描述的凯尔表达改变的例子不同。先证者的表型在19个月内一直未变，这表明它不是一种短暂的情况。然而，家族研究没有提供证据表明它是可遗传的。通过免疫沉淀从先证者的红细胞中分离出一种93-kD蛋白，该蛋白与兔抗凯尔蛋白抗体在蛋白质印迹法中反应较弱。在随后的研究中未能重现这一发现，这表明回收的凯尔蛋白数量处于所用技术可检测的阈值水平。该红细胞表型被归类为Kmod，这是首次在健康个体中报道的此类例子。
