Orford C R, Gardner D L
Connect Tissue Res. 1984;12(3-4):345-8. doi: 10.3109/03008208409013696.
Proteoglycans of canine articular cartilage were labelled for transmission electron microscopy using the cationic copper phthalocyanin dye, cupromeronic blue, in a critical electrolyte concentration method. Much of the proteoglycan appeared to be structurally unrelated to collagen but a small proportion was positioned close to fibrils. On demonstrating the characteristic collagen banding pattern with uranyl acetate and phosphotungstic acid, it was evident that proteoglycan interacted with collagen at the d band.
采用临界电解质浓度法，使用阳离子铜酞菁染料（亚甲蓝）对犬关节软骨蛋白聚糖进行标记，用于透射电子显微镜观察。大部分蛋白聚糖在结构上似乎与胶原蛋白无关，但有一小部分位于靠近纤维的位置。在用醋酸铀和磷钨酸显示出特征性的胶原条纹图案时，很明显蛋白聚糖在d带与胶原蛋白相互作用。
