Orford C R, Gardner D L
Histochem J. 1985 Feb;17(2):223-33. doi: 10.1007/BF01003221.
Two collagen-poor, ultramicroscopic layers are described at the surface of canine articular cartilage. They are distinguished by staining with an electron-dense cationic dye, Cupromeronic Blue, in a critical electrolyte concentration technique and by digestion with testicular hyaluronidase. The superficial layer, approximately 50 nm thick, stained at low electrolyte concentrations but failed to stain in conditions specific for sulphated glycosaminoglycans. It was hyaluronidase-resistant and may be either glycoprotein or protein in nature. The deeper layer, 100-400 nm thick, stained positively at electrolyte concentrations specific for sulphated glycosaminoglycans but not in conditions specific for keratan sulphate. It was removed by hyaluronidase digestion. This layer probably represents a chondroitin sulphate-rich proteoglycan. These surface layers may be important in the lubrication of the articular surface and in the permeability and compression resistance of the superficial cartilage zone.
在犬关节软骨表面描述了两个胶原含量低的超微层。它们通过在临界电解质浓度技术中用电子致密阳离子染料铜铬黑蓝染色以及用睾丸透明质酸酶消化来区分。表层约50纳米厚，在低电解质浓度下染色，但在硫酸化糖胺聚糖特有的条件下不染色。它对透明质酸酶有抗性，本质上可能是糖蛋白或蛋白质。较深层厚100 - 400纳米，在硫酸化糖胺聚糖特有的电解质浓度下呈阳性染色，但在硫酸角质素特有的条件下不染色。它通过透明质酸酶消化被去除。这一层可能代表富含硫酸软骨素的蛋白聚糖。这些表面层可能在关节表面的润滑以及表层软骨区的通透性和抗压性方面很重要。
