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用纯化的HLA I类基因转化LMTK-细胞。VI. HLA - B7和AW24分子的血清学特征

Transformation of LMTK- cells with purified HLA class I gene. VI. Serological characterization of HLA-B7 and AW24 molecules.

作者信息

Layet C, Le Bouteiller P P, N'guyen C, Mercier P, Rosa F, Fellous M, Caillol D H, Jordan B R, Lemonnier F A

出版信息

Hum Immunol. 1984 Sep;11(1):31-45. doi: 10.1016/0198-8859(84)90054-5.

DOI:10.1016/0198-8859(84)90054-5
PMID:6207154
Abstract

Serological characterization of HLA-B7 and HLA-AW24 class I molecules following transfection of murine LMTK- cells with purified HLA class I genes was performed using human alloantisera. Induction by murine alpha interferon of the expression of class I molecules was required to obtain unambiguous identification of these molecules which appear serologically identical to the HLA-B7 and HLA-AW24 molecules expressed at the surface of human peripheral blood lymphocytes of 20 unrelated individuals. Analysis of the transformed cells with 8 different anti-HLA class I monoclonal antibodies results in the definition of 3 separate clusters of antigenic determinants shared by all HLA class I molecules. These studies further suggest the existence of locus-specific serological reactivities associated either with the HLA-A or with the HLA-B and C gene products.

摘要

在用纯化的HLA I类基因转染小鼠LMTK-细胞后，使用人同种异体抗血清对HLA-B7和HLA-AW24 I类分子进行了血清学鉴定。为了明确鉴定这些在血清学上与20名无关个体外周血淋巴细胞表面表达的HLA-B7和HLA-AW24分子相同的分子，需要用鼠α干扰素诱导I类分子的表达。用8种不同的抗HLA I类单克隆抗体对转化细胞进行分析，确定了所有HLA I类分子共有的3个独立的抗原决定簇簇。这些研究进一步表明，存在与HLA-A或与HLA-B和C基因产物相关的位点特异性血清学反应性。

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引用本文的文献

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Immunogenetics. 1994;40(4):306-8. doi: 10.1007/BF00189978.
2
Serological expression after sequential double transfection with purified HLA-A11 gene of mouse fibroblasts carrying human beta-2 microglobulin.携带人β2微球蛋白的小鼠成纤维细胞经纯化的HLA - A11基因连续两次转染后的血清学表达。
Immunogenetics. 1985;22(1):1-8. doi: 10.1007/BF00430589.
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The HLA-AW24 gene: sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes.
HLA - AW24基因：序列、周边情况以及与HLA - A2和HLA - A3基因的比较
Immunogenetics. 1985;21(5):479-89. doi: 10.1007/BF00430931.
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Expression of an HLA-Bw6-related specificity by the HLA-Cw3 molecule.HLA - Cw3分子表达与HLA - Bw6相关的特异性。
Immunogenetics. 1985;21(5):469-78. doi: 10.1007/BF00430930.
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Antigenic determinants shared between HLA-A, -B, -C antigens and H-2 class I molecules modified by bovine beta-2 microglobulin.HLA - A、- B、- C抗原与经牛β2微球蛋白修饰的H - 2 I类分子之间共有的抗原决定簇。
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