N'Guyen C, Sodoyer R, Trucy J, Strachan T, Jordan B R
Immunogenetics. 1985;21(5):479-89. doi: 10.1007/BF00430931.
A cosmid clone containing two class I sequences was found to cause expression of the HLA-AW24 protein after transfection into mouse L cells. The restriction map of this cosmid shows extensive homology over 26 kb with the map of the HLA-A3 region obtained from cosmids of the same library, constructed with DNA from an HLA-A3/HLA-AW24 heterozygote, but diverges over the remaining 14 kb. The HLA-AW24 gene was subcloned from this cosmid and its nucleotide sequence was determined. Amino acid and, more strikingly, nucleotide sequence comparisons with other HLA alleles indicate that the A locus alleles are more closely related to each other than to alleles from other HLA loci. A very skewed distribution of silent substitutions is apparent, and the occurrence of clustered multiple substitutions hints at gene-conversion-like events.
发现一个含有两个I类序列的黏粒克隆在转染到小鼠L细胞后可导致HLA - AW24蛋白的表达。该黏粒的限制酶切图谱显示，在26 kb范围内与从同一文库的黏粒获得的HLA - A3区域图谱具有广泛的同源性，该文库是用来自HLA - A3/HLA - AW24杂合子的DNA构建的，但在其余14 kb范围内存在差异。从该黏粒中克隆出HLA - AW24基因并测定了其核苷酸序列。与其他HLA等位基因的氨基酸序列比较，更显著的是核苷酸序列比较表明，A位点等位基因彼此之间的关系比与其他HLA位点的等位基因更为密切。沉默替代的分布非常不均衡，成簇的多个替代的出现暗示了类似基因转换的事件。
