Singhal R P
J Chromatogr. 1983 Aug 26;266:359-83. doi: 10.1016/s0021-9673(01)90909-4.
A survey of recent advances in high-performance liquid chromatography (HPLC) of tRNA is presented here. The polystyrene and reversed-phase anion exchangers are discussed for their ability to resolve tRNAs without loss of the aminoacyl-tRNA bond. The HPLC of a tRNA of choice, based on the affinity principle, is studied. Both chemical (boronate) and biological (plant lectins) affinity groups for the tRNA interaction are described. A comprehensive scheme is presented for the separation of four mammalian tRNAs. Scope of future research in this area is also discussed.
本文介绍了对转运核糖核酸（tRNA）高效液相色谱（HPLC）近期进展的一项调查。讨论了聚苯乙烯和反相阴离子交换剂在不损失氨酰tRNA键的情况下分离tRNA的能力。研究了基于亲和原理对选定tRNA进行的HPLC分析。描述了用于tRNA相互作用的化学（硼酸酯）和生物（植物凝集素）亲和基团。提出了一个分离四种哺乳动物tRNA的综合方案。还讨论了该领域未来的研究范围。
