Sokolski K N, Lechago J
J Histochem Cytochem. 1984 Oct;32(10):1066-74. doi: 10.1177/32.10.6207221.
Human colonic mucosa was immunostained with antibodies against substance P to identify the endocrine cells containing this peptide in the mucosal glands. Dual immunohistochemical and histochemical studies were also carried out to determine whether these cells are enterochromaffin cells and contain serotonin as claimed in the literature. The results obtained indicate that the normal human colonic substance P-producing cells are not argentaffin cells, nor do they contain serotonin. In addition, they are also negative both for several silver and for other techniques commonly used to identify digestive endocrine cells. They are positive, however, with the argyrophilic technique of Churukian-Schenk. It is concluded, therefore, that the substance P-producing cells of the human colonic mucosa are not a subpopulation of the enterochromaffin cells, but constitute a distinct and independent cell type.
用人结肠黏膜与抗P物质抗体进行免疫染色，以鉴定黏膜腺体内含有该肽的内分泌细胞。还进行了双重免疫组织化学和组织化学研究，以确定这些细胞是否如文献中所述为肠嗜铬细胞并含有5-羟色胺。所得结果表明，正常人类结肠中产生P物质的细胞不是嗜银细胞，也不含有5-羟色胺。此外，它们对几种银染法以及常用于鉴定消化内分泌细胞的其他技术均呈阴性。然而，它们对Churukian-Schenk嗜银技术呈阳性。因此得出结论，人类结肠黏膜中产生P物质的细胞不是肠嗜铬细胞的一个亚群，而是构成一种独特且独立的细胞类型。
