Wetzel G D, Swain S L, Dutton R W, Kettman J R
J Immunol. 1984 Nov;133(5):2327-32.
The sites and modes of action of several B cell mitogens and interleukins were examined. Cell cycle analyses of B cell responses to several polyclonal activators including LPS, DXS, LPS plus DXS, and anti-immunoglobulin were performed. Two different states of B cell activation distinguished by RNA content, DNA content, and cell size were observed. LPS promoted transitions throughout the cell cycle, whereas DXS primarily caused exit from G0. Synergy between LPS and DXS was observed in elicitation of exit from G0. Activation by anti-immunoglobulin was found to be influenced by the antibody dose and the cell density of culture. Interleukins could influence anti-IgM-induced responses by increasing G0 exit, and by increasing commitment to DNA synthesis. A model of B cell activation in which cells are stimulated to a stage with intermediate RNA levels (G1A) by polyclonal activators is suggested. Some interleukins appear to be involved in this process. At G1A, cells are receptive to signals delivered by interleukins or some polyclonal activators that drive them to late G1 and DNA synthesis. After cell division, cells re-enter G1A in which interleukins or mitogens are necessary for a continued response.
对几种B细胞促有丝分裂原和白细胞介素的作用位点及作用方式进行了研究。对B细胞对包括脂多糖（LPS）、二硫苏糖醇（DXS）、LPS加DXS以及抗免疫球蛋白在内的几种多克隆激活剂的反应进行了细胞周期分析。观察到了两种由RNA含量、DNA含量和细胞大小区分的不同B细胞激活状态。LPS促进细胞在整个细胞周期中的转变，而DXS主要导致细胞从G0期退出。在诱导细胞从G0期退出方面观察到LPS和DXS之间的协同作用。发现抗免疫球蛋白激活受抗体剂量和培养细胞密度的影响。白细胞介素可通过增加G0期退出以及增加对DNA合成的投入来影响抗IgM诱导的反应。提出了一种B细胞激活模型，其中多克隆激活剂将细胞刺激到具有中等RNA水平（G1A）的阶段。一些白细胞介素似乎参与了这一过程。在G1A期，细胞对白细胞介素或一些多克隆激活剂传递的信号敏感，这些信号将它们驱动到G1晚期和DNA合成。细胞分裂后，细胞重新进入G1A期，在这个阶段白细胞介素或促有丝分裂原对于持续反应是必需的。
