Gu J, Blank M A, Huang W M, Islam K N, McGregor G P, Christofides N, Allen J M, Bloom S R, Polak J M
Urology. 1984 Oct;24(4):353-7. doi: 10.1016/0090-4295(84)90209-7.
Nerves containing immunoreactive vasoactive intestinal polypeptide (VIP), substance P and two newly discovered peptides, neuropeptide tyrosine (NPY) and PHI (peptide having N-terminal histidine and C-terminal isoleucine), have been found in the human urinary bladder by immunocytochemistry and radioimmunoassay. Somatostatin immunoreactivity was detected by radioimmunoassay. The VIP-immunoreactive nerves were widely distributed in all regions, but were particularly dense beneath the epithelium and in the muscle layer. Scattered intramural ganglia were found to be reactive to VIP antiserum. Higher concentrations of extractable VIP were detected in the trigone than in the dome. VIP- and PHI-immunoreactive nerves were similarly distributed, the latter being less numerous. NPY-immunoreactive nerves were seen mainly in the muscle layer, particularly in the trigonal area. The distribution patterns of VIP- and NPY-immunoreactive nerves resembled those of the previously reported cholinergic and adrenergic nerves, respectively. Many blood vessels were found to be innervated by both types of immunoreactive nerves. Scattered substance P-immunoreactive fibers were occasionally seen, being present in the submucosa and around the detrusor muscles. The significance of these nerves remains to be elucidated.
通过免疫细胞化学和放射免疫测定法，在人类膀胱中发现了含有免疫反应性血管活性肠肽（VIP）、P物质以及两种新发现的肽——神经肽Y（NPY）和PHI（具有N端组氨酸和C端异亮氨酸的肽）的神经。通过放射免疫测定法检测到生长抑素免疫反应性。VIP免疫反应性神经广泛分布于所有区域，但在上皮下方和肌层中尤为密集。发现散在的壁内神经节对VIP抗血清有反应。三角区可提取的VIP浓度高于穹窿部。VIP和PHI免疫反应性神经分布相似，后者数量较少。NPY免疫反应性神经主要见于肌层，尤其是三角区。VIP和NPY免疫反应性神经的分布模式分别类似于先前报道的胆碱能神经和肾上腺素能神经。发现许多血管受两种免疫反应性神经支配。偶尔可见散在的P物质免疫反应性纤维，存在于黏膜下层和逼尿肌周围。这些神经的意义尚待阐明。
