Department of Clinical Diagnostics, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-957, Olsztyn, Poland.
Agri Plus Sp. Z O.O., Marcelinska Street 92, 60-324, Poznań, Poland.
Sci Rep. 2021 Jun 9;11(1):12147. doi: 10.1038/s41598-021-91529-0.
Bisphenol A (BPA) is used in the production of plastics approved for contact with feed and food. Upon entering living organisms, BPA, as a potent endocrine disruptor, negatively affects various internal organs and regulatory systems, especially in young individuals. Although previous studies have described the neurotoxic effects of BPA on various tissues, it should be underlined that the putative influence of this substance on the chemical architecture of the urinary bladder intrinsic innervation has not yet been studied. One of the most important neuronal substances involved in the regulation of urinary bladder functions is vasoactive intestinal polypeptide (VIP), which primarily participates in the regulation of muscular activity and blood flow. Therefore, this study aimed to determine the influence of various doses of BPA on the distribution pattern of VIP-positive neural structures located in the wall of the porcine urinary bladder trigone using the double-immunofluorescence method. The obtained results show that BPA influence leads to an increase in the number of both neurons and nerve fibres containing VIP in the porcine urinary bladder trigone. This may indicate that VIP participates in adaptive processes of the urinary bladder evoked by BPA.
双酚 A（BPA）用于生产获准与饲料和食品接触的塑料。进入生物体后，BPA 作为一种有效的内分泌干扰物，会对各种内部器官和调节系统产生负面影响，尤其是在年轻个体中。尽管先前的研究已经描述了 BPA 对各种组织的神经毒性作用，但应该强调的是，尚未研究这种物质对膀胱内在神经支配的化学结构的可能影响。参与调节膀胱功能的最重要的神经元物质之一是血管活性肠肽 (VIP)，它主要参与调节肌肉活动和血液流动。因此，本研究旨在使用双重免疫荧光法确定不同剂量的 BPA 对位于猪膀胱三角区壁的 VIP 阳性神经结构分布模式的影响。所得结果表明，BPA 影响导致猪膀胱三角区中含有 VIP 的神经元和神经纤维数量增加。这可能表明 VIP 参与了由 BPA 引起的膀胱适应性过程。
