Heinle H, Lindner V
Arch Int Physiol Biochim. 1984 Apr;92(1):13-7. doi: 10.3109/13813458409073408.
The binding of Evans blue to collagen and elastin in rabbit aortic tissue and in bovine ligamentum nuchae was studied following circulation in vivo of the dye and incubation in vitro in Evans blue containing plasma, respectively. Using collagenase and elastase, the dye was liberated from both tissues corresponding to their different contents of collagen and elastin. Disc electrophoretic analysis of the liberated dye showed, that it migrated as free Evans blue indicating that the binding of the dye to the macromolecules was due to spatial interactions rather than to fixation at specific prosthetic groups. The capability of collagen and elastin to bind Evans blue was demonstrated with the isolated proteins; it was shown elastin had a higher affinity to the dye than collagen. Treatment of the blued tissue with hyaluronidase and Triton X-100 showed that binding to complex carbohydrates and dye accumulation in the aqueous intra- or extracellular space seems to be negligible.
分别在染料体内循环后以及在含伊文思蓝的血浆中体外孵育后，研究了伊文思蓝与兔主动脉组织和牛项韧带中胶原蛋白和弹性蛋白的结合情况。使用胶原酶和弹性蛋白酶，根据两种组织中胶原蛋白和弹性蛋白含量的不同，从两种组织中释放出染料。对释放出的染料进行圆盘电泳分析表明，它以游离伊文思蓝的形式迁移，这表明染料与大分子的结合是由于空间相互作用，而不是固定在特定的辅基上。用分离出的蛋白质证明了胶原蛋白和弹性蛋白结合伊文思蓝的能力；结果表明弹性蛋白对染料的亲和力高于胶原蛋白。用透明质酸酶和 Triton X - 100处理染蓝的组织表明，与复合碳水化合物的结合以及染料在细胞内或细胞外水相空间中的积累似乎可以忽略不计。
