Proffitt R, Case R M
Cell Calcium. 1984 Aug;5(4):335-50. doi: 10.1016/0143-4160(84)90002-2.
Orthovanadate accelerates 45Ca efflux and enzyme secretion from the rat pancreas incubated in either control (2.5 mM Ca) or nominally Ca-free buffers. Secretion induced by vanadate does not appear to be mediated by changes in either adenylate cyclase or sodium pump activity. Instead, vanadate appears to act at an intracellular site to cause the release of calcium from the same pool mobilised by acetylcholine. Vanadate action is not inhibited by DIDS. The effect of pH on vanadate action may be accounted for by changes in the distribution of the vanadates. Vanadyl sulphate inhibits secretion evoked by acetylcholine. This suggests that intracellular reduction of vanadate (+5 oxidation state) to the +4 oxidation state may account for an inhibitory component observed during stimulation with vanadate.
原钒酸盐可加速在对照（2.5 mM钙）或名义上无钙缓冲液中孵育的大鼠胰腺的45Ca外流和酶分泌。钒酸盐诱导的分泌似乎不是由腺苷酸环化酶或钠泵活性的变化介导的。相反，钒酸盐似乎作用于细胞内位点，导致从与乙酰胆碱动员的相同钙库中释放钙。钒酸盐的作用不受DIDS抑制。pH对钒酸盐作用的影响可能是由钒酸盐分布的变化引起的。硫酸氧钒抑制乙酰胆碱诱发的分泌。这表明钒酸盐（+5氧化态）在细胞内还原为+4氧化态可能解释了在用钒酸盐刺激期间观察到的抑制成分。
