Murthy V V, Karmen A
Department of Laboratory Medicine, Albert Einstein College of Medicine, Bronx, NY 10461.
Clin Chem. 1990 Mar;36(3):559-61.
We evaluated the CEDIA digoxin immunoassay (Microgenics, Inc., Concord, CA), as performed with the Cobas-Bio centrifugal analyzer. In assays of sera with known concentrations of digoxin, the enzyme activity, measured when two beta-galactosidase (EC 3.2.1.23) fragments were combined according to the assay format, was proportional to the digoxin concentration. Results of assays of sera containing 0 to 3 micrograms of digoxin per liter correlated well when compared with an RIA method: CEDIA, microgram/L = 1.00 x RIA - 0.06 microgram/L (n = 90, r = 0.95, Sxy = 0.05). Duplicate assays of three control sera containing 0.8, 2.2, or 3.3 micrograms/L, each analyzed 20 times a day with each group of patients' samples, gave within-run CVs of 1-3% and day-to-day CVs of 3-12%. The reconstituted CEDIA reagents were stable for at least a month at 5 degrees C. As many as 25 samples and controls can be assayed in half the time needed to complete a similar number of RIA measurements with comparable results.
我们评估了使用Cobas - Bio离心分析仪进行的CEDIA地高辛免疫分析（Microgenics公司，加利福尼亚州康科德）。在对已知地高辛浓度的血清进行分析时，按照分析形式将两个β - 半乳糖苷酶（EC 3.2.1.23）片段结合时所测量的酶活性与地高辛浓度成正比。与放射免疫分析（RIA）方法相比，每升含0至3微克地高辛的血清分析结果相关性良好：CEDIA（微克/升）= 1.00×RIA - 0.06微克/升（n = 90，r = 0.95，Sxy = 0.05）。对含0.8、2.2或3.3微克/升的三种对照血清进行重复分析，每组患者样本每天分析20次，批内变异系数为1 - 3%，日间变异系数为3 - 12%。重构的CEDIA试剂在5摄氏度下至少稳定一个月。与完成类似数量的RIA测量所需时间相比，完成多达25个样本和对照的分析所需时间减半，且结果相当。
