妊娠相关血浆蛋白-A的酶免疫测定
Enzyme immunoassay for pregnancy-associated plasma protein-A.
作者信息
MacDonald D J, Mehta H C, Mack D S, Duffy T, Glen A C
出版信息
Clin Chem. 1984 Nov;30(11):1848-50.
PMID:6207960
Abstract
This enzyme immunoassay procedure for pregnancy-associated plasma protein type A involves a "sandwich"-type system in microtitration plates. One can detect 0.27 mg of the protein per liter of serum, with a between-batch CV of 10.2%, and the antiserum used does not cross react with any of the other placentally derived proteins. A reference interval for the last trimester of pregnancy is presented. The procedure described is suitable for studying the behavior of this protein during pregnancy.
摘要
这种用于妊娠相关血浆蛋白A的酶免疫测定方法采用微量滴定板中的“夹心”型系统。每升血清中可检测到0.27毫克该蛋白，批间变异系数为10.2%，所用抗血清与任何其他胎盘衍生蛋白均无交叉反应。给出了妊娠晚期的参考区间。所描述的方法适用于研究该蛋白在孕期的变化情况。
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