Hamilton R G, Adkinson N F
Crit Rev Clin Lab Sci. 1984;21(1):1-18. doi: 10.3109/10408368409165803.
The diagnosis and management of human allergic disease is aided by serological measurements involving the quantitation of total human serum IgE, allergen-specific IgE and allergen-specific IgG. Initially, a brief overview of immediate hypersensitivity reactions will be presented with a focus on the role of "reaginic" allergen-specific IgE antibody. The principles and design of immunoassay methods employed in the measurement of total serum IgE and allergen-specific human IgE will then be examined with a discussion of the pros and cons of different radioallergosorbent test (RAST) reporting schemes. Next, Hymenoptera venom will be used as a model allergen system for exploring the clinical utility, principles and design of immunoassays used to quantitate allergen-specific IgG antibody in human serum. Examples of the utility of specific IgG measurements will be provided with recent clinical data. Finally, an overview will be presented on more research-based techniques used in the study of human allergic responses such as cross-immunoelectrophoresis and immunoassays for leukotrienes, prostaglandins, platelet Factor 4 and cyclic AMP.
人类过敏性疾病的诊断和管理借助于血清学检测，这些检测涉及人血清总IgE、过敏原特异性IgE和过敏原特异性IgG的定量。首先，将简要概述速发型超敏反应，重点关注“反应素性”过敏原特异性IgE抗体的作用。然后将研究用于测量血清总IgE和过敏原特异性人IgE的免疫测定方法的原理和设计，并讨论不同放射变应原吸附试验（RAST）报告方案的优缺点。接下来，将以膜翅目毒液作为模型过敏原系统，探讨用于定量人血清中过敏原特异性IgG抗体的免疫测定的临床应用、原理和设计。将提供特定IgG测量值效用的示例以及近期临床数据。最后，将概述用于研究人类过敏反应的更多基于研究的技术，如交叉免疫电泳以及白三烯、前列腺素、血小板因子4和环磷酸腺苷的免疫测定。
