Vendrell J P, Huguet M F, Cannat A, Serre A
Dev Biol Stand. 1984;56:221-6.
PI, a lipid A-free fraction (15) extracted from the cell wall of Brucella melitensis type I M.15 behaves as a non specific mitogen and a polyclonal B-cell activator (PBA) for human unsensitized lymphocytes from subjects with no previous history and no biological stigmata of brucellosis. Both effects may be evidenced, indifferently, in cultures of peripheral blood, tonsils or cord blood lymphocytes. Mitogenicity, estimated by the incorporation of tritiated thymidine is maximal on the 5th and 6th day of culture, for high cellular concentrations and for 10 micrograms/PI/well. PBA activity is estimated by the incidence of Prot. A reverse PFC and by a laser nephelometric measure of immunoglobulins in culture supernatants. The use of ET-rosetting for the preparation of purified and/or reconstituted populations demonstrates the T-dependence of the B cell activating effect of PI. On the other hand adherent cell depletion on Sephadex G 10 columns does not significantly impair these effects. Important individual differences are observed and suggest a genetic control of the level of B responses to this new bacterial PBA. These results are discussed in terms of a comparison of PI with other human PBA and of the interactions between non specific and specific responses of lymphocytes from vaccinated subjects or brucellosis patients.
PI是从I型羊种布鲁氏菌M.15细胞壁中提取的无脂质A组分（15），对于既往无布鲁氏菌病病史和生物学体征的未致敏人淋巴细胞，它表现为一种非特异性有丝分裂原和多克隆B细胞激活剂（PBA）。在外周血、扁桃体或脐血淋巴细胞培养物中，均可同等地证实这两种效应。通过掺入氚标记的胸腺嘧啶核苷来估计的有丝分裂原性，在培养的第5天和第6天，对于高细胞浓度和10微克/PI/孔时达到最大值。PBA活性通过蛋白A反向PFC的发生率以及通过激光散射比浊法测量培养上清液中的免疫球蛋白来估计。使用红细胞玫瑰花结法制备纯化和/或重组群体表明PI的B细胞激活作用依赖于T细胞。另一方面，在葡聚糖凝胶G 10柱上耗尽贴壁细胞并不会显著损害这些效应。观察到重要的个体差异，提示对这种新型细菌PBA的B细胞反应水平存在遗传控制。根据PI与其他人类PBA的比较以及来自接种疫苗的受试者或布鲁氏菌病患者的淋巴细胞非特异性和特异性反应之间的相互作用对这些结果进行了讨论。
