Smith S, Halling S M
Gene. 1984 Sep;29(3):263-9. doi: 10.1016/0378-1119(84)90055-6.
Cloned DNA fragments encoding portions of canine parvovirus (CPV) structural proteins were inserted into plasmid expression vectors. These plasmids expressed CPV-beta-galactosidase fusion proteins under the transcriptional control of the Escherichia coli lac promoter-operator. The fusion proteins were purified and used to immunize rabbits. Rabbit antibodies raised against these fusion proteins were shown to immunoprecipitate authentic CPV structural proteins from infected cell extracts. This demonstrated that the CPV-beta-galactosidase fusion proteins expressed in bacteria elicit antibodies which can recognize determinants of authentic CPV proteins. However, none of the antibodies neutralizes CPV virus particles.
将编码犬细小病毒（CPV）结构蛋白部分片段的克隆DNA片段插入质粒表达载体。这些质粒在大肠杆菌乳糖启动子-操纵子的转录控制下表达CPV-β-半乳糖苷酶融合蛋白。融合蛋白被纯化并用于免疫兔子。针对这些融合蛋白产生的兔抗体可从感染细胞提取物中免疫沉淀出真正的CPV结构蛋白。这表明在细菌中表达的CPV-β-半乳糖苷酶融合蛋白能引发可识别真正CPV蛋白决定簇的抗体。然而，这些抗体均不能中和CPV病毒颗粒。
