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犬用犬细小病毒重组疫苗。

Recombinant vaccine for canine parvovirus in dogs.

作者信息

López de Turiso J A, Cortés E, Martínez C, Ruiz de Ybáñez R, Simarro I, Vela C, Casal I

机构信息

INGENASA, Madrid, Spain.

出版信息

J Virol. 1992 May;66(5):2748-53. doi: 10.1128/JVI.66.5.2748-2753.1992.

DOI:10.1128/JVI.66.5.2748-2753.1992
PMID:1313899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC241030/
Abstract

VP2 is the major component of canine parvovirus (CPV) capsids. The VP2-coding gene was engineered to be expressed by a recombinant baculovirus under the control of the polyhedrin promoter. A transfer vector that contains the lacZ gene under the control of the p10 promoter was used in order to facilitate the selection of recombinants. The expressed VP2 was found to be structurally and immunologically indistinguishable from authentic VP2. The recombinant VP2 shows also the capability to self-assemble, forming viruslike particles similar in size and appearance to CPV virions. These viruslike particles have been used to immunize dogs in different doses and combinations of adjuvants, and the anti-CPV responses have been measured by enzyme-linked immunosorbent assay, monolayer protection assays, and an assay for the inhibition of hemagglutination. A dose of ca. 10 micrograms of VP2 was able to elicit a good protective response, higher than that obtained with a commercially available, inactivated vaccine. The results indicate that these viruslike particles can be used to protect dogs from CPV infection.

摘要

VP2是犬细小病毒（CPV）衣壳的主要成分。VP2编码基因经改造后由重组杆状病毒在多角体蛋白启动子的控制下表达。为便于筛选重组体，使用了一种在p10启动子控制下含有lacZ基因的转移载体。发现表达的VP2在结构和免疫学上与天然VP2没有区别。重组VP2还具有自我组装的能力，能形成大小和外观与CPV病毒粒子相似的病毒样颗粒。这些病毒样颗粒已被用于以不同剂量和佐剂组合免疫犬，并且通过酶联免疫吸附测定、单层保护测定和血凝抑制测定来测量抗CPV反应。约10微克的VP2剂量能够引发良好的保护性反应，高于市售灭活疫苗所产生的反应。结果表明，这些病毒样颗粒可用于保护犬免受CPV感染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e89/241030/204e299441be/jvirol00037-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e89/241030/a0be7edf885b/jvirol00037-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e89/241030/204e299441be/jvirol00037-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e89/241030/a0be7edf885b/jvirol00037-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e89/241030/204e299441be/jvirol00037-0163-a.jpg

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