Morinet F, D'Auriol L, Tratschin J D, Galibert F
Service de Bacteriologie et Virologie, Centre Hayem, Hôpital Saint-Louis, Paris, France.
J Gen Virol. 1989 Nov;70 ( Pt 11):3091-7. doi: 10.1099/0022-1317-70-11-3091.
A 1.4 kb fragment (nucleotides 2430 to 3901) encoding portions of the human parvovirus B19 structural proteins was inserted into the pRIT2 plasmid expression vector containing the gene encoding staphylococcal Protein A under the control of the phage lambda promoter PR. The fusion protein was used to raise antibodies in rabbits. The sera were shown by immune electron microscopy to agglutinate B19 particles and were also shown to recognize the VP2 B19 capsid protein, by Western blot analysis. The B19 antigenicity of the fusion protein was confirmed by immunoblot and enzyme immunoassay with IgG and IgM anti-B19-positive reference human sera.
将一段编码人细小病毒B19结构蛋白部分序列的1.4 kb片段（核苷酸2430至3901）插入到pRIT2质粒表达载体中，该载体含有在噬菌体λ启动子PR控制下编码葡萄球菌蛋白A的基因。用该融合蛋白在兔体内产生抗体。免疫电镜显示血清可凝集B19颗粒，Western印迹分析表明血清还能识别B19衣壳蛋白VP2。用IgG和IgM抗B19阳性参考人血清进行免疫印迹和酶免疫测定，证实了融合蛋白的B19抗原性。
