Ishii M, Furukawa M, Okada M, Hamada T
J Cutan Pathol. 1984 Aug;11(4):292-5. doi: 10.1111/j.1600-0560.1984.tb00380.x.
Improved ruthenium red staining has been applied to normal human skin and lichen myxoedematosus skin lesions for the detection of the fine structure proteoglycan aggregates. In 4 samples of normal skin and in a patient with lichen myxoedematosus, the typical fine ultrastructures of proteoglycan aggregates, i.e., many short irregularly-curled filaments of varying lengths attached to long and rather straight filaments of hyaluronic acid were shown. Although lichen myxoedematosus is thought to be due to the faulty formation of proteoglycan aggregates, we suggest a different view, that this improved method of ruthenium red staining is useful for the detection of proteoglycan aggregates, and provides a better preparation for electron microscopy.
改良的钌红染色法已应用于正常人体皮肤和黏液水肿性苔藓皮肤病变，以检测蛋白聚糖聚集体的精细结构。在4份正常皮肤样本和1例黏液水肿性苔藓患者中，均显示出蛋白聚糖聚集体典型的精细超微结构，即许多长短不一的短而不规则卷曲的细丝附着于透明质酸的长且相对直的细丝上。尽管黏液水肿性苔藓被认为是由于蛋白聚糖聚集体形成缺陷所致，但我们提出了不同的观点，即这种改良的钌红染色法有助于检测蛋白聚糖聚集体，并为电子显微镜检查提供了更好的标本制备。
