Moore S, Richardson M
Atherosclerosis. 1985 Jun;55(3):313-30. doi: 10.1016/0021-9150(85)90109-1.
The aortic lesions induced in normal-fed rabbits by an indwelling catheter were examined for changes in lipid content shown by oil red O-stained sections and associated proteoglycan distribution and ultrastructural changes by transmission electron microscopy of ruthenium red-stained sections, during an 8-week period of regression. Compared to normal aorta there was a highly significant increase in proteoglycan in lipid-containing lesions, which was also associated with the presence of regenerated endothelium. In the lesions which had regressed in terms of size and lipid content, the proteoglycan concentration, especially in superficial regions, was significantly reduced compared to early lesions and was similar to that seen in the normal vessel. Proteoglycan concentration decreased before lipid content of lesions was reduced. Proteoglycan was not associated with lipid-containing macrophages. These observations support the hypothesis that an increased glycosaminoglycan concentration is associated with lipid deposition in the vessel wall in response to injury in the absence of hyperlipaemia.
在为期8周的消退期内，通过油红O染色切片检查正常喂养兔留置导管诱导的主动脉病变的脂质含量变化，通过钌红染色切片的透射电子显微镜检查相关蛋白聚糖分布和超微结构变化。与正常主动脉相比，含脂质病变中的蛋白聚糖显著增加，这也与再生内皮的存在有关。在大小和脂质含量方面已经消退的病变中，与早期病变相比，蛋白聚糖浓度，尤其是在浅表区域，显著降低，与正常血管中的浓度相似。病变脂质含量降低之前，蛋白聚糖浓度就已下降。蛋白聚糖与含脂质巨噬细胞无关。这些观察结果支持这样的假设，即在无高脂血症的情况下，糖胺聚糖浓度增加与血管壁损伤后脂质沉积有关。
