Sharma H C, Gill B S
Stain Technol. 1984 Jul;59(4):197-200. doi: 10.3109/10520298409113856.
The Feulgen procedure was applied to chromosome preparations of leaf meristems from growing plants of wheat, barley, and wheat-wheatgrass hybrids. Leaf primordia from the base of secondary tillers were pretreated in cold water (overnight, 2 C), fixed in glacial acetic acid (20 min, 2 C), hydrolyzed in 1 N HC1 (14 min), stained in leuco-basic fuchsin (about 15 min) and squashed in 1% acetocarmine. The chromosome spreads from leaf meristems were generally superior to those of the root meristems of the same plant. Mitotic index in leaf meristems was higher than in root meristems in some species. The method appears useful for counting the chromosome number of growing plants, detecting chimeras, and verifying root tip chromosome counts.
将福尔根染色法应用于小麦、大麦以及小麦-冰草属杂种生长植株叶片分生组织的染色体标本制备。取自二级分蘖基部的叶原基先在冷水中预处理（2℃过夜），然后用冰醋酸固定（2℃，20分钟），再在1N盐酸中水解（14分钟），用无色碱性品红染色（约15分钟），最后在1%醋酸洋红中压片。叶片分生组织的染色体铺片通常优于同一植株的根分生组织。在某些物种中，叶片分生组织的有丝分裂指数高于根分生组织。该方法似乎有助于对生长植株进行染色体计数、检测嵌合体以及验证根尖染色体计数结果。
