Kraus J E, de Sousa H C, Rezende M H, Castro N M, Vecchi C, Luque R
Botany Department, IB-USP, São Paulo-SP, Brazil.
Biotech Histochem. 1998 Sep;73(5):235-43. doi: 10.3109/10520299809141117.
Methods for double staining plant materials using astra blue and basic fuchsin are described here. These methods can be applied to free hand and microtome sections embedded in paraffin, paraplast or historesin. Also, they can be used to study isolated epidermal peels and pollen preparations. Temporary, semipermanent and permanent preparations were studied. Astra blue stained polysaccharides of the cell wall such as cellulose and pectins. Basic fuchsin showed an affinity for lignified, suberized or cutinized walls. The easy preparation of the reagents, excellent color contrast of the histological preparations, and brief staining times of some methods makes them useful for both routine research and didactic purposes. Also, excellent color or black and white photomicrography can be obtained after the double staining described here.
本文描述了使用阿斯特拉蓝和碱性品红对植物材料进行双重染色的方法。这些方法可应用于徒手切片以及包埋在石蜡、石蜡塑料或组织树脂中的切片。此外，它们还可用于研究分离的表皮切片和花粉制剂。对临时、半永久和永久制剂进行了研究。阿斯特拉蓝可对细胞壁的多糖如纤维素和果胶进行染色。碱性品红对木质化、栓质化或角质化的细胞壁具有亲和力。试剂制备简便、组织学制剂的颜色对比极佳以及某些方法的染色时间较短，使其对常规研究和教学目的均有用处。此外，经过此处描述的双重染色后，可获得出色的彩色或黑白显微照片。
