Levings R L, Kaeberle M L, Reed D E
Vet Microbiol. 1984 Aug;9(4):329-44. doi: 10.1016/0378-1135(84)90002-6.
Bovine embryonic kidney cells were infected with bovine herpesviruses (BHV1, 2, or 3), suid herpesvirus 1 (SHV1), or were sham-inoculated. When cytopathic effect was apparent, the cells were solubilized using Triton X-100 detergent. Resulting antigen preparations were tested by 2-dimensional immunoelectrophoresis using bovine fetal serum and antisera directed against BHV1, BHV2, BHV3, SHV1 or a restricted spectrum of BHV1 antigens. Interaction of BHV1 antiserum with BHV1 antigen preparations resulted in 11 precipitation arcs. The same antiserum produced 3 arcs with BHV2, none with BHV3, and 5 with SHV1. The interaction of BHV1 antigen preparations with BHV2, BHV3, or SHV1 antisera failed to produce demonstrable arcs. However, when heterologous antigen or antibody preparations were added to BHV1 homologous 2-dimensional immunoelectrophoresis tests, all 11 BVH1 arcs were modified by BHV1, 2 by BHV2, 4 by BHV3 and 4 by SHV1 preparations. Two antigens were common to the 4 herpesviruses. Antigen preparations were tested for their ability to inhibit virus neutralization by BHV1 antiserum; only the BHV1 preparation was active. Sera were tested for BHV1 neutralizing activity; only BHV1 antiserum and a serum specific for a restricted spectrum of BHV1 antigens were active. A glycoprotein antigen associated with BHV1 neutralization was identified which may be important in the protection of animals against disease.
用牛疱疹病毒（BHV1、2或3）、猪疱疹病毒1（SHV1）感染牛胚胎肾细胞，或进行假接种。当细胞病变效应明显时，用曲拉通X-100去污剂溶解细胞。使用牛胎血清以及针对BHV1、BHV2、BHV3、SHV1或BHV1抗原受限谱的抗血清，通过二维免疫电泳对所得抗原制剂进行检测。BHV1抗血清与BHV1抗原制剂相互作用产生了11条沉淀弧。同一抗血清与BHV2产生3条弧，与BHV3无弧产生，与SHV1产生5条弧。BHV1抗原制剂与BHV2、BHV3或SHV1抗血清相互作用未能产生可证实的弧。然而，当将异源抗原或抗体制剂添加到BHV1同源二维免疫电泳试验中时，所有11条BHV1弧都被BHV1、2条被BHV2、4条被BHV3和4条被SHV1制剂修饰。4种疱疹病毒有2种共同抗原。检测抗原制剂抑制BHV1抗血清病毒中和的能力；只有BHV1制剂具有活性。检测血清的BHV1中和活性；只有BHV1抗血清和一种针对BHV1抗原受限谱的特异性血清具有活性。鉴定出一种与BHV1中和相关的糖蛋白抗原，其可能在保护动物免受疾病侵害方面具有重要作用。
