Levings R L, Kaeberle M L, Reed D E
Vet Microbiol. 1984 Aug;9(4):313-28. doi: 10.1016/0378-1135(84)90001-4.
Bovine embryonic kidney cells were infected with bovine herpesvirus 1 (BHV1) or were sham-inoculated. When cytopathic effect was apparent, the cells were treated with beta-propiolactone, formalin, heat (56 degrees C), or ultraviolet irradiation until the virus was inactivated. Infected-treated, infected-untreated (IU) and sham-inoculated cultures were solubilized using Triton X-100 detergent. Resulting preparations were tested by 2-dimensional- and fused rocket-immunoelectrophoresis and were evaluated for their ability to inhibit virus neutralization by BHV1 antiserum. Eleven viral antigens were detected consistently in IU preparations, which strongly inhibited virus neutralization. Eight or more IU antigens were detected in beta-propiolactone-treated, formalin-treated and heat-treated preparations; these inhibited virus neutralization less strongly than the IU preparations. No IU antigens were detected in ultraviolet-treated preparations, nor did this material inhibit virus neutralization. One of the IU antigens was reduced preferentially by all treatments. The selective destruction of antigens by the various treatments might allow antigen-specific serological testing to distinguish vaccinated from naturally-exposed cattle.
牛胚胎肾细胞用牛疱疹病毒1型（BHV1）感染或进行假接种。当细胞病变效应明显时，用β-丙内酯、福尔马林、加热（56℃）或紫外线照射处理细胞，直至病毒灭活。用Triton X-100去污剂溶解经感染处理、未处理的感染细胞（IU）和假接种的培养物。用二维和融合火箭免疫电泳对所得制剂进行检测，并评估其抑制BHV1抗血清中和病毒的能力。在IU制剂中始终检测到11种病毒抗原，这些抗原强烈抑制病毒中和。在经β-丙内酯处理、福尔马林处理和加热处理的制剂中检测到8种或更多的IU抗原；这些制剂抑制病毒中和的能力不如IU制剂强。在经紫外线处理的制剂中未检测到IU抗原，该材料也不抑制病毒中和。所有处理均优先减少一种IU抗原。各种处理对抗原的选择性破坏可能使抗原特异性血清学检测能够区分接种疫苗的牛和自然感染的牛。
