Joshi N J, Nandedkar T D
Acta Endocrinol (Copenh). 1984 Oct;107(2):268-74. doi: 10.1530/acta.0.1070268.
Human chorionic gonadotrophin (hCG)-like activity has been reported in mouse and rabbit blastocysts. The presence of this hCG-like activity seems to be essential for nidation of the pre-implantation embryo. Binding sites for hCG were localised on day 4 mouse embryos by immunohistochemical techniques. Presence of hCG-like activity was confirmed by cytotoxicity test. The number of implantation sites was significantly reduced on day 8 of pregnancy. Prior treatment to day 4 mouse embryos with hCG antiserum, for 1 h in utero or in vitro and their subsequent transfer to uteri of synchronised pseudopregnant mice resulted in impaired implantation of embryos, compared to controls treated with normal rabbit serum (NRS). These results suggest that hCG-like activity present on the pre-implantation embryo may have a significant role in implantation of the embryo.
在小鼠和兔的囊胚中已报道有人绒毛膜促性腺激素（hCG）样活性。这种hCG样活性的存在似乎对植入前胚胎的着床至关重要。通过免疫组织化学技术将hCG的结合位点定位在第4天的小鼠胚胎上。通过细胞毒性试验证实了hCG样活性的存在。妊娠第8天着床部位的数量显著减少。与用正常兔血清（NRS）处理的对照组相比，在子宫内或体外对第4天的小鼠胚胎用hCG抗血清处理1小时，随后将其转移到同期假孕小鼠的子宫中，导致胚胎着床受损。这些结果表明，植入前胚胎上存在的hCG样活性可能在胚胎着床中起重要作用。
