Khan-Dawood F S, Dawood M Y
Am J Obstet Gynecol. 1984 Feb 15;148(4):359-65. doi: 10.1016/0002-9378(84)90707-5.
To determine the temporal relationship between immunoreactive chorionic gonadotropin, chorionic gonadotropin receptors, and implantation of the rabbit blastocyst, (1) immunoreactive chorionic gonadotropin in plasma, uterine flushings and blastocysts; (2) chorionic gonadotropin receptors on blastocysts (day 5 and 6) and embryonic and interembryonic segments of the uterus (day 7); and (3) chorionic gonadotropin receptors on the endometrium and myometrium (day 1 through 6) were measured. Binding of I125-labeled beta-subunit of human chorionic gonadotropin (hCG) by cell membranes from blastocysts increased significantly from 6.0 +/- 1.1 fmol/mg of protein (mean +/- SE) on day 5 (N = 6) to 16.1 +/- 1.3 fmol/mg of protein on day 6 (n = 6) (P less than 0.001). Immunoreactive chorionic gonadotropin levels in blastocyst fluid increased from 0.3 ng/ml of day 5 to 0.65 ng/ml on day 6. Specific binding of I125-labeled hCG was absent in endometrial and myometrial cell membranes before implantation (days 1 to 6) but was found in decidual cells from embryonic segments on day 7. Plasma immunoreactive chorionic gonadotropin or chorionic gonadotropin--like material increased from 6 ng/ml of day 1 to 52 ng/ml on day 7. Uterine flushings had chorionic gonadotropin levels of 0.4 ng/ml of day 2, which increased slowly to 1.0 ng/ml on day 7. Intrauterine instillation of hCG into nonpregnant uterine horns demonstrated transuterine absorption of hCG with plasma hCG levels showing a dose-related response. Our findings demonstrate that (1) immunoreactive chorionic gonadotropin or chorionic gonadotropin--like material is detectable in plasma, uterine flushings, and blastocyst fluid before implantation, (2) chorionic gonadotropin receptors are present on the blastocyst cells before implantation, and (3) the uterus can absorb chorionic gonadotropin from its lumen.
为确定免疫反应性绒毛膜促性腺激素、绒毛膜促性腺激素受体与兔胚泡着床之间的时间关系，对以下各项进行了测定：（1）血浆、子宫冲洗液和胚泡中的免疫反应性绒毛膜促性腺激素；（2）胚泡（第5天和第6天）以及子宫的胚胎段和胚外段（第7天）上的绒毛膜促性腺激素受体；（3）子宫内膜和子宫肌层（第1天至第6天）上的绒毛膜促性腺激素受体。胚泡细胞膜对I125标记的人绒毛膜促性腺激素（hCG）β亚基的结合量从第5天（N = 6）的6.0±1.1 fmol/mg蛋白质（平均值±标准误）显著增加至第6天（n = 6）的16.1±1.3 fmol/mg蛋白质（P＜0.001）。胚泡液中的免疫反应性绒毛膜促性腺激素水平从第5天的0.3 ng/ml增加至第6天的0.65 ng/ml。着床前（第1天至第6天），子宫内膜和子宫肌层细胞膜上不存在I125标记的hCG特异性结合，但在第7天胚胎段的蜕膜细胞中可检测到。血浆免疫反应性绒毛膜促性腺激素或类绒毛膜促性腺激素物质从第1天的6 ng/ml增加至第7天的52 ng/ml。子宫冲洗液中绒毛膜促性腺激素水平在第2天为0.4 ng/ml，至第7天缓慢增加至1.0 ng/ml。向未孕子宫角内子宫内滴注hCG显示hCG可经子宫吸收，血浆hCG水平呈现剂量相关反应。我们的研究结果表明：（1）着床前血浆、子宫冲洗液和胚泡液中可检测到免疫反应性绒毛膜促性腺激素或类绒毛膜促性腺激素物质；（2）着床前胚泡细胞上存在绒毛膜促性腺激素受体；（3）子宫可从其管腔吸收绒毛膜促性腺激素。
