Syrjänen K, Syrjänen S, Pyrhönen S
ORL J Otorhinolaryngol Relat Spec. 1982;44(6):323-34. doi: 10.1159/000275612.
Paraffin sections from 36 laryngeal squamous cell carcinomas with histological features of coexistent condylomatous changes (the flat, inverted and papillomatous lesions caused by human papilloma virus; HPV) were stained for HPV antigens with an indirect immunoperoxidase technique. The HPV antiserum used was prepared in guinea pigs immunized against the purified HPV virions from human common wart tissue. Of the tumors studied, 36% showed the presence of HPV antigens. In that, one-third of the flat and inverted lesions and 42% of the papillomatous lesions contained cells with intranuclear HPV-staining reaction. The role of HPV as the etiologic agent of laryngeal squamous cell papillomas is discussed, as is the potential malignant transformation of them. Based on the present results, the previously expressed view on the probable role of HPV in the development of laryngeal squamous cell carcinoma seems to gain further substantiation.
对36例具有共存尖锐湿疣样改变（由人乳头瘤病毒；HPV引起的扁平、内翻和乳头状病变）组织学特征的喉鳞状细胞癌石蜡切片，采用间接免疫过氧化物酶技术对HPV抗原进行染色。所用的HPV抗血清是在豚鼠中制备的，这些豚鼠用来自人寻常疣组织的纯化HPV病毒粒子免疫。在所研究的肿瘤中，36%显示存在HPV抗原。其中，三分之一的扁平及内翻病变和42%的乳头状病变含有具有核内HPV染色反应的细胞。讨论了HPV作为喉鳞状细胞乳头状瘤病因的作用，以及它们潜在的恶性转化。基于目前的结果，先前关于HPV在喉鳞状细胞癌发生中可能作用的观点似乎得到了进一步证实。
