Moreau N, Lacroix P, Fournel L
Anal Biochem. 1984 Aug 15;141(1):94-100. doi: 10.1016/0003-2697(84)90430-5.
Polyethyleneglycol/dextran and polyethyleneglycol/salt two-phase systems are used to measure the entry of antibiotics into Escherichia coli and Staphylococcus aureus cells. Aminoglycosides, macrolides, a quinolone, and a cycline were assayed. The method is simple and rapid and eliminates the problems encountered with filters, especially nonspecific binding; it allows rapid uptake kinetics to be measured.
聚乙二醇/葡聚糖和聚乙二醇/盐双相系统用于测定抗生素进入大肠杆菌和金黄色葡萄球菌细胞的情况。对氨基糖苷类、大环内酯类、一种喹诺酮类和一种四环素类进行了分析。该方法简单快速，消除了使用滤膜时遇到的问题，尤其是非特异性结合问题；它能够测定快速摄取动力学。
