青枯雷尔氏菌无毒菌株（M4S）脂多糖O抗原的结构研究。

Structural studies of the O-antigen of the lipopolysaccharide from an avirulent strain (M4S) of Pseudomonas solanacearum.

作者信息

Akiyama Y, Eda S, Kato K, Tanaka H

出版信息

Carbohydr Res. 1984 Oct 15;133(2):289-96. doi: 10.1016/0008-6215(84)85205-2.

PMID:6209012

Abstract

The structure of the O-antigen of the lipopolysaccharide from an avirulent strain (M4S) of Pseudomonas solanacearum has been investigated by methylation analysis, n.m.r. spectroscopy, and N-deacetylation-deamination, followed by analysis and controlled Smith-degradation of the product. These studies demonstrate that the O-antigen is composed of a tetrasaccharide repeating-unit having the following structure: ----3)-alpha-D-GlcpNAc-(1----2)-alpha-L-Rhap-(1----2)-alpha- L-Rhap-(1----3)- alpha-L-Rhap-(1----.

摘要

通过甲基化分析、核磁共振光谱以及N-脱乙酰化-脱氨基作用，随后对产物进行分析和可控的史密斯降解，研究了青枯雷尔氏菌无毒菌株（M4S）脂多糖O抗原的结构。这些研究表明，O抗原由具有以下结构的四糖重复单元组成：----3)-α-D-氨基葡萄糖-(1----2)-α-L-鼠李糖-(1----2)-α-L-鼠李糖-(1----3)-α-L-鼠李糖-(1---- 。