Lenglet W J, Bos A J, vd Stap C C, Vis R D, Delhez H, vd Hamer C J
Histochemistry. 1984;81(3):305-9. doi: 10.1007/BF00495645.
This paper contains a comparison between four experimental methods for the detection of trace metals in biological tissues, e.g. autoradiography, dithizone-, Timm-staining and microPIXE (Particle Induced X-ray Emission). As research object the hippocampus of the rat was chosen and mainly the Zn-distribution in the fascia dentata has been examined. The comparison was judged on four criteria: spatial resolution, specificity, quantification and influence of the protein binding of the metal on the outcome of each method. Some additional Timm and microPIXE experiments have been performed to compare the metal concentrations for both normal and Zn-deficient rats. The Zn/Cu ratio for both kinds of rats were calculated and some striking differences in the concentrations were obtained. The Zn/Cu ratio for a normal rat was found to be about 40% higher than for a deficient one.
本文对生物组织中痕量金属检测的四种实验方法进行了比较，例如放射自显影法、双硫腙染色法、蒂姆染色法和微束质子激发X射线发射分析（PIXE）法。选用大鼠海马体作为研究对象，主要检测了齿状回中锌的分布情况。比较基于四个标准进行：空间分辨率、特异性、定量分析以及金属蛋白结合对每种方法结果的影响。另外还进行了一些蒂姆染色法和微束质子激发X射线发射分析实验，以比较正常大鼠和缺锌大鼠的金属浓度。计算了两种大鼠的锌/铜比率，结果发现浓度存在显著差异。正常大鼠的锌/铜比率比缺锌大鼠高约40%。
