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相似文献

1
Mapping nucleolar proteins with monoclonal antibodies.用单克隆抗体绘制核仁蛋白图谱。
J Cell Biol. 1984 Dec;99(6):1981-8. doi: 10.1083/jcb.99.6.1981.
2
Characterization of the nucleolar protein, B-36, using monoclonal antibodies.
Exp Cell Res. 1986 Sep;166(1):77-93. doi: 10.1016/0014-4827(86)90509-4.
3
Monoclonal antibody characterization of the C proteins of heterogeneous nuclear ribonucleoprotein complexes in vertebrate cells.脊椎动物细胞中异质性核糖核蛋白复合物C蛋白的单克隆抗体特性分析
J Cell Biol. 1984 Dec;99(6):1997-204. doi: 10.1083/jcb.99.6.1997.
4
Immunolocalization of the 100 kDa nucleolar protein during the mitotic cycle in CHO cells.CHO细胞有丝分裂周期中100 kDa核仁蛋白的免疫定位
Biol Cell. 1985;53(3):209-18. doi: 10.1111/j.1768-322x.1985.tb00369.x.
5
The small nuclear ribonucleoproteins that react with anti-Sm and anti-RNP antibodies.与抗Sm抗体和抗RNP抗体发生反应的小核核糖核蛋白。
Biochem Biophys Res Commun. 1984 Aug 30;123(1):345-51. doi: 10.1016/0006-291x(84)90419-4.
6
A monoclonal antibody (HNo-g7) with specificity for a human nucleolar protein.
Biosci Rep. 1984 Sep;4(9):789-95. doi: 10.1007/BF01128821.
7
Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies.利用单克隆抗体对体内异质核核糖核蛋白复合物进行表征。
Mol Cell Biol. 1984 Jun;4(6):1104-14. doi: 10.1128/mcb.4.6.1104-1114.1984.
8
Alterations in immunolocalization of the phosphoprotein B23 in HeLa cells during serum starvation.血清饥饿期间HeLa细胞中磷蛋白B23免疫定位的变化。
Exp Cell Res. 1985 Nov;161(1):101-10. doi: 10.1016/0014-4827(85)90494-x.
9
The effect of harmine on the localization of the nucleolar proteins C23 and B23.骆驼蓬碱对核仁蛋白C23和B23定位的影响。
Eur J Cell Biol. 1985 May;37:213-5.
10
Identification and characterization of a hexameric form of nucleolar phosphoprotein B23.
Biochim Biophys Acta. 1987 Jul 16;925(1):74-82. doi: 10.1016/0304-4165(87)90149-8.

引用本文的文献

1
Global functional analysis of nucleophosmin in Taxol response, cancer, chromatin regulation, and ribosomal DNA transcription.核磷蛋白在紫杉醇反应、癌症、染色质调控和核糖体DNA转录中的全局功能分析。
Exp Cell Res. 2007 Jan 1;313(1):65-76. doi: 10.1016/j.yexcr.2006.09.016. Epub 2006 Sep 26.
2
Nucleolar localization of the Werner syndrome protein in human cells.沃纳综合征蛋白在人细胞中的核仁定位。
Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):6887-92. doi: 10.1073/pnas.95.12.6887.
3
N-band proteins of nucleolar organizers: chromosomal mapping, subnucleolar localization and rDNA binding.核仁组织区的N带蛋白：染色体定位、核仁亚定位及rDNA结合
Chromosoma. 1986;93(3):231-42. doi: 10.1007/BF00292743.
4
Identification of a 70,000-D protein in lens membrane junctional domains.晶状体膜连接结构域中一种70,000道尔顿蛋白质的鉴定。
J Cell Biol. 1985 Jul;101(1):28-35. doi: 10.1083/jcb.101.1.28.
5
A constitutive nucleolar protein identified as a member of the nucleoplasmin family.一种被鉴定为核质蛋白家族成员的组成型核仁蛋白。
EMBO J. 1987 Jul;6(7):1881-90. doi: 10.1002/j.1460-2075.1987.tb02447.x.
6
Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein.一种与大鼠肝脏核仁蛋白相似的酵母核仁蛋白的鉴定与特性分析。
J Cell Biol. 1988 Jul;107(1):17-31. doi: 10.1083/jcb.107.1.17.
7
Immunohistochemical and biochemical characterization of antisera raised to human tumor nucleoli.针对人肿瘤核仁产生的抗血清的免疫组织化学和生化特性分析
J Cancer Res Clin Oncol. 1989;115(4):351-60. doi: 10.1007/BF00400962.
8
A protein of Mr 80,000 is associated with the nucleolus organizer of human cell lines.一种分子量为80,000的蛋白质与人类细胞系的核仁组织者相关。
Chromosoma. 1986;94(5):353-61. doi: 10.1007/BF00328635.

本文引用的文献

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Isolation and characterization of a high-molecular-weight acid-soluble nuclear protein from mouse ascites-sarcoma cells.
Eur J Biochem. 1980 Jul;108(2):497-505. doi: 10.1111/j.1432-1033.1980.tb04747.x.
2
Higher order metaphase chromosome structure: evidence for metalloprotein interactions.高阶中期染色体结构：金属蛋白相互作用的证据。
Cell. 1982 May;29(1):171-81. doi: 10.1016/0092-8674(82)90101-5.
3
Evidence for two levels of DNA folding in histone-depleted HeLa interphase nuclei.在组蛋白缺失的HeLa间期细胞核中存在两级DNA折叠的证据。
J Mol Biol. 1982 Apr 5;156(2):309-24. doi: 10.1016/0022-2836(82)90331-x.
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Localization of phosphoprotein C23 to nucleolar structures and to the nucleolus organizer regions.磷蛋白C23在核仁结构和核仁组织区的定位。
Exp Cell Res. 1981 Nov;136(1):101-9. doi: 10.1016/0014-4827(81)90041-0.
5
A simple method of reducing the fading of immunofluorescence during microscopy.一种减少显微镜检查期间免疫荧光褪色的简单方法。
J Immunol Methods. 1981;43(3):349-50. doi: 10.1016/0022-1759(81)90183-6.
6
Nucleolar localization of protein B23 (37/5.1) by immunocytochemical techniques.
Life Sci. 1981 Mar 23;28(12):1371-9. doi: 10.1016/0024-3205(81)90411-2.
7
Non-histone proteins and long-range organization of HeLa interphase DNA.非组蛋白与海拉细胞间期DNA的长程组织
J Mol Biol. 1982 Apr 5;156(2):325-44. doi: 10.1016/0022-2836(82)90332-1.
8
Distribution of proteins among chromatin components of nucleoli.蛋白质在核仁染色质成分中的分布。
Biochemistry. 1983 Jun 21;22(13):3187-93. doi: 10.1021/bi00282a023.
9
Localization of phosphoprotein C23 in nucleoli by immunological methods.通过免疫学方法对核仁中磷蛋白C23进行定位。
Exp Cell Res. 1981 Oct;135(2):259-65. doi: 10.1016/0014-4827(81)90161-0.
10
Interaction of nucleolar phosphoprotein C23 with cloned segments of rat ribosomal deoxyribonucleic acid.核仁磷蛋白C23与大鼠核糖体脱氧核糖核酸克隆片段的相互作用。
Biochemistry. 1983 Jul 5;22(14):3345-51. doi: 10.1021/bi00283a007.

用单克隆抗体绘制核仁蛋白图谱。

Mapping nucleolar proteins with monoclonal antibodies.

作者信息

Kistler J, Duncombe Y, Laemmli U K

出版信息

J Cell Biol. 1984 Dec;99(6):1981-8. doi: 10.1083/jcb.99.6.1981.

DOI:10.1083/jcb.99.6.1981
PMID:6209284
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2113565/
Abstract

Using monoclonal antibodies as probes, we have characterized three antigens with respect to localization in the nucleolus, molecular weight and solubility. Two proteins, of 110,000 and 94,000 apparent molecular weight, were found associated with the ribonucleoprotein fibers. A third protein, with a molecular weight of 40,000, was accumulated at the nucleolar periphery, was present in the nucleoplasm, and may be involved in pre-ribosome maturation and transport.

摘要

我们使用单克隆抗体作为探针，从核仁定位、分子量和溶解性方面对三种抗原进行了表征。发现两种表观分子量分别为110,000和94,000的蛋白质与核糖核蛋白纤维相关。第三种蛋白质分子量为40,000，聚集在核仁周边，存在于核质中，可能参与核糖体前体的成熟和运输。